Catalog No: AB2000 Reactivity: H, R, M, Mk, Ch, Ze, Fish
Isotype: Rabbit IgG Applications: WB
UniProt ID: P04406
All Names: aging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685
Form: Liquid
Storage instructions: Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer: pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity: Affinity-chromatography
Immunogen: A synthesized peptide
Molecular Wt.: 36 kDa
WB 1:5000~1:20000
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.

Western blot analysis of GAPDH expression in
(1) Helacell lysate;
(2)Jurkat cell lysate;
(3)Mouse kidney lysate;
(4) Mouse spleen lysate;
(5) RAW 264.7 cell lysate;
(6)Rat brain lysate with GAPDH Antibody.